
Title
Accounting for Systematic Variation in Difference Gel Electrophoresis
Speaker
Kimberly Sellers, Carnegie Mellon University
Abstract
Two-dimensional Difference Gel Electrophoresis (DIGE) circumvents many of the problems associated with gel comparison via the traditional approach, two-dimensional gel electrophoresis (2DE). DIGE's accuracy, however, can be further improved by removing the many associated sources of systematic variation. In this talk, I will identify these sources (including those caused by the apparatus detection system for locating proteins) and illustrate the results using data from a collection of experiments.