Title
Temporal Profile Study of Gene Expression for Female Rainbow Trout Under Normal and Compressed Cycles
Speaker
Yushi Liu, The Ohio State University
Abstract
New statistical procedures are introduced to investigate gene activity in support of the hypothalamus-pituitary-gonad-liver (HPGL) signaling network that provides the neuroendocrine regulation for reproduction in female, oviparous fishes. The methods include SCOOP (Shrunken Centroid Ordering by Orthogonal Projections) and a robust encoding of B-splines via Friedman’s Generalized Elastic Net (GEN). Clustering of genes via GEN-transformed measurements produced much more stable results, and the mean time course pattern of each cluster provided biologists with a reliable summary from which to interpret systematic patterns. Ultimately, the genes selected by SCOOP and clustered though the GEN-transform strongly suggested supportive pathways involving immunology, muscle contraction, reproduction, protein transport, metabolism and reduction/oxidation.
